Messi had missed the weekend’s Ligue 1 draw against Stade de Reims in which PSG failed to score in the league for the first time this season.
The 35-year-old had asked to be withdrawn in the second half of the reverse fixture at Benfica last week due to a calf problem.
Presnel Kimpembe, Nuno Mendes and Renato Sanches will also miss Tuesday’s game.
PSG are top of Group H at the halfway stage with seven points, the same as Benfica, while Juventus are four points behind in third.
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